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Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation
Heimes, A. Brodhagen, J. Weikard, R. Becker, D. Meyerholz, M. M. Petzl, W. Zerbe, H. Schuberth, H. J. Hoedemaker, M. Schmicke, M.
Heimes, A.
Brodhagen, J.
Weikard, R.
Becker, D.
Meyerholz, M. M.
Petzl, W.
Zerbe, H.
Schuberth, H. J.
Hoedemaker, M.
Schmicke, M.
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Issue Date
2020-07-01
Submitted date
2019-09-19
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Abstract
Infection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Association
Citation
Heimes A, Brodhagen J, Weikard R, Becker D, Meyerholz MM, Petzl W, Zerbe H, Schuberth HJ, Hoedemaker M, Schmicke M, Engelmann S, Kühn C. Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation. J Dairy Sci. 2020 Jul;103(7):6364-6373. doi: 10.3168/jds.2019-17612. Epub 2020 Apr 16. PMID: 32307160.
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en
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Infection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region. © 2020 American Dairy Science Association
Series/Report no.
Volume 103, Issue 7, July 2020, Pages 6364-6373
ISSN
00220302
EISSN
15253198
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Landwirtschaftliche Rentenbank
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Attribution-NonCommercial 4.0 International