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Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.
Gurramkonda, Chandrasekhar Polez, Sulena Skoko, Natasa Adnan, Ahmad Gäbel, Thomas Chugh, Dipti Swaminathan, Sathyamangalam Khanna, Navin Tisminetzky, Sergio Rinas, Ursula
Gurramkonda, Chandrasekhar
Polez, Sulena
Skoko, Natasa
Adnan, Ahmad
Gäbel, Thomas
Chugh, Dipti
Swaminathan, Sathyamangalam
Khanna, Navin
Tisminetzky, Sergio
Rinas, Ursula
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Abstract
The prevalence of diabetes is predicted to rise significantly in the coming decades. A recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries.
Citation
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin. 2010, 9:31 Microb. Cell Fact.
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Journal
PubMed ID
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Article
Language
en
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Series/Report no.
ISSN
1475-2859