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BIOCHEMICAL CHARACTERIZATION OFA HIGHLY SPECIFIC TRIMETHYLAMINE DEHYDROGENASE SUITED FOR THE APPLICATION IN BIOSENSORS

Hummel, W.
; 
Wendel, U.
; 
Sting, Susanne
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Issue Date
1992
Submitted date
2024-05-08
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Abstract
A strain of Paracoccus possessing a NAD (P)*-independent trimethylamine dehydrogenase could be isolated by enrichment cultivation. The enzyme was purified up to 4.2 U/mg. Phenazine ethosulfate acts as an electron acceptor, other artificial mediators such as methylene blue or K3[Fe(CN)¢] are inactive. Important biochemical data are: pH-optimum: 9.0; substrates: specific for trimethylamine; dimethylamine, methylamine or $rimethylamine-N-oxide are not converted; Ky(trimethylamine): 6.6*¢10°° M. The calibration curve for trimethylamine (aqueous solution) using a kinetic photometric assay with dichlorophenol-indophenol shows a linearity between 0.1 - 0.8 uM trimethylamine.
Citation
Biosensors : fundamentals, technologies and applications, 381 - 384
Affiliation
Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf; Forschungszentrum Jülich, Pf 2050, D-5170 Jülich; Kinderklinik der Heinrich-Heine-Universität Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623823
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Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International
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