Thumbnail Image
Publication

Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung.

Kordes, Adrian
; 
Preusse, Matthias
; 
Willger, Sven D
; 
Braubach, Peter
; 
Jonigk, Danny
; 
Haverich, Axel
; 
Warnecke, Gregor
; 
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2019-07-30
Submitted date
Show all metadata
Files
Thumbnail Image
Open Access publication
Adobe PDF, 1.64 MB
Other Titles
Abstract
Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic variation, the ex vivo transcriptional profiles of P. aeruginosa populations inhabiting different regions of the CF lung are similar. Although we cannot estimate the extent to which the transcriptional response recorded here actually reflects the in vivo transcriptomes, our results indicate that there may be a common in vivo transcriptional profile in the CF lung environment.
Citation
Nat Commun. 2019 Jul 30;10(1):3397. doi: 10.1038/s41467-019-11414-3.
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Springer-Nature
Journal
Nature Communications
URI
http://hdl.handle.net/10033/621898
DOI
10.1038/s41467-019-11414-3
PubMed ID
31363089
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
2041-1723
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
openAccess
Attribution-NonCommercial-ShareAlike 4.0 International
cbn
Collections
publications of the research group of molecular bacteriology(MOBA)