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FLOW INJECTION IMMUNOANALYSIS (FIIA) FOR THE DETERMINATION OF PESTICIDES IN WATER
Stöcklein, W. Krämer, P. Schmid, Rolf D.
Stöcklein, W.
Krämer, P.
Schmid, Rolf D.
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
The principle of a heterogeneous competitive enzyme immunoassay was applied to a flow injection system. This flow injection immunoassay (FIIA) can be run automatically. Antibodies to the triazine herbicide atrazin were immobilized on membranes. Antibody containing membranes were mounted in a cross-flow reactor and changed after each assay. In the assay atrazin competed with an atrazin-peroxidase conjugate for the antibody binding sites. The product of the enzyme reaction was measured fluorimetrically. One assay is completed within 15 minutes. Sensitivities of the FIIA proved similar to the ELISA and depended mainly on the properties of the antibodies and conjugation strategies. The potential and limitations of the FIIA system will be discussed.
Citation
Biosensors : applications in medicine, environmental protection and process control, 307 - 312
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International