Loading...
TOTAL SYNTHESIS OF YEAST ALANYL trNa
Wang, Debao
Wang, Debao
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
1987
Submitted date
2023-04-19
Files
Loading...
PDF
Adobe PDF, 6.27 MB
Other Titles
Abstract
By means of a combination of chemical and enzymatic methods, we have synthesized an RNA molecule which has the same chemical composition (i.e. it contains all the common as well as the modified nucleotides) and structure as the natural yeast alanyl tRNA, elucidated by R.W. Holley et.al. and modified by others, and which is biologically active (1). In the presence of rat liver aminoacyl-tRNA synthetase, the synthetic tRNA can be charged with alanine and the incorporation of alanine from the charged tRNA molecule into proteins is readily demonstrated in a rabbit reticulocyte ‘ lysate system.
Citation
Chemical synthesis in molecular biology, 43 ff
Publisher
DOI
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF Monographs, Vol. 8
ISSN
0930-4320
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International