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LIPASES IN REVERSE MICELLES
Walde, Peter Luisi, Pier Luigi
Walde, Peter
Luisi, Pier Luigi
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Issue Date
1991
Submitted date
2024-03-20
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Abstract
Two independent spectroscopic methods have been developed to assay lipases continuously with triacylglycerol substrates in a reverse micellar solution. With the two methods, a simple and unique possibility is offered to study the kinetics and the specificity of lipases, embedded in a system which possibly mimics the biologically relevant environment of lipolytic enzymes. Preliminary activity data are presented for the colipase dependent human pancreatic lipase in reverse micelles, and we have started to investigate the conformational behavior of this enzyme by means of circular dichroism and fluorescence spectroscopy.
Citation
Lipases : structure, mechanism and genetic engineering, 155 - 158
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International