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DETECTION OF LIGANDS VIA BACTERIAL LUCIFERASE
Jockers, Ralf Schmid, Rolf D.
Jockers, Ralf
Schmid, Rolf D.
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Issue Date
1992
Submitted date
2024-04-17
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Abstract
Bacterial luciferase is a widely applied enzyme in medical diagnosis. The applications harness the FMNH,-dependency of the luciferase. Via FMNH,it is possible to couple other FMN-dependent enzymes or enzyme systems. With the help of FMN/NADHoxidoreductase coupling with NAD/NADH-dependent enzymes and enzymesystems it is possible to broaden the range of application”. luciferase FMNH,+ O, + aldehyde ------------------ » FMN + H,O + acid + light The amount of emitted luminescent light is proportional to the substrates and can be easily monitored with a luminometer. In addition to FMNH,, other substrates are converted, for example an aldehyde into the corresponding acid. Very low concentrations of these aldehydes can be detected and the reaction has a large linear range (5 to 100000 nM). Maximal activity (luminescence) is achieved through nalkanes with a chain length of C-10 to 14. Hereit will be shown that modified aldehydes are as effective substrates as unmodified ones. Furthermore, the interaction of both will be characterized and a general scheme of ligand detection presented.
Citation
Biosensors : fundamentals, technologies and applications, 235 - 237
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International