Loading...
An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo.
Jenke, Bok Hee C Fetzer, Christian P Stehle, Isa M Jönsson, Franziska Fackelmayer, Frank O Conradt, Harald Bode, Jürgen Lipps, Hans J
Jenke, Bok Hee C
Fetzer, Christian P
Stehle, Isa M
Jönsson, Franziska
Fackelmayer, Frank O
Conradt, Harald
Bode, Jürgen
Lipps, Hans J
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2002-04
Submitted date
Other Titles
Abstract
pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class.
Citation
An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo. 2002, 3 (4):349-54 EMBO Rep.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1469-221X