Loading...
Capsule and fimbriae modulate the invasion of Haemophilus influenzae in a human blood-cerebrospinal fluid barrier model.
Häuser, Svenja Wegele, Christian Stump-Guthier, Carolin Borkowski, Julia Weiss, Christel Rohde, Manfred Ishikawa, Hiroshi Schroten, Horst Schwerk, Christian Adam, Rüdiger
Häuser, Svenja
Wegele, Christian
Stump-Guthier, Carolin
Borkowski, Julia
Weiss, Christel
Rohde, Manfred
Ishikawa, Hiroshi
Schroten, Horst
Schwerk, Christian
Adam, Rüdiger
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2018-07-17
Submitted date
Files
Loading...
original manuscript
Adobe PDF, 422.96 KB
Loading...
supplementary materials
Adobe PDF, 144.83 MB
Loading...
figures 1 to 6
Adobe PDF, 97.63 MB
Other Titles
Abstract
The Gram-negative bacterium Haemophilus influenzae (H. influenzae) can commensally colonize the upper respiratory tract, but also cause life threatening disease including epiglottitis, sepsis and meningitis. The H. influenzae capsule protects the bacteria against both phagocytosis and opsonization. Encapsulated H. influenzae strains are classified into serotypes ranging from a to f dependent on their distinct polysaccharide capsule. Due to the implementation of vaccination the incidence of invasive H. influenzae type b (Hib) infections has strongly decreased and infections with other capsulated types, including H. influenzae type f (Hif), are emerging. The pathogenesis of H. influenzae meningitis is not clarified. To enter the central nervous system (CNS) the bacteria generally have to cross either the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BSCFB). Using a cell culture model of the BCSFB based on human choroid plexus papilloma (HIBCPP) cells and different H. influenzae strains we investigated whether Hib and Hif invade the cells, and if invasion differs between encapsulated vs. capsular-deficient and fimbriated vs. non-fimbriated variants. We find that Hib can adhere to and invade into HIBCPP cells. Invasion occurs in a strongly polar fashion, since the bacteria enter the cells preferentially from the basolateral "blood "side. Fimbriae and capsule attenuate invasion into choroid plexus (CP) epithelial cells, and capsulation can influence the bacterial distribution pattern. Finally, analysis of clinical Hib and Hif isolates confirms the detected invasive properties of H. influenzae. Our data point to roles of capsule and fimbriae during invasion of CP epithelial cells.
Citation
Int J Med Microbiol. 2018 Oct;308(7):829-839. doi: 10.1016/j.ijmm.2018.07.004. Epub 2018 Jul 17.
Publisher
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
EISSN
1618-0607
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International