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Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3

Kuzhyk, Yuriy
; 
Lopatniuk, Maria
; ; 
Fedorenko, Victor
; 
Ostash, Bohdan
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Issue Date
2018-09-25
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Abstract
Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.
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Affiliation
HIPS, Helmholtz-Institut für Pharmazeutische Forschung Saarland, Universitätscampus E8.1 66123 Saarbrücken, Germany.
Publisher
Springer
Journal
Indian Journal of Microbiology
URI
http://hdl.handle.net/10033/621641
DOI
10.1007/s12088-018-0761-x
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Additional Links
http://link.springer.com/10.1007/s12088-018-0761-x
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Article
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ISSN
0046-8991
0973-7715
0-7084-0623-8
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Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the research group metabolic engineering of Actinomycetes ([HIPS]AMEG)