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CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF A LIPASE FROM A SPECIES OF PSEUDOMONAS
Sarma, Ragupathy Dauberman, Judy Poulose, A. J. Van Bielen, Jan Power, Scott Shew, Brian Gray, Greg Norton, Shirley Van Kimmenade, Anita Jarnagin, Alisha
Sarma, Ragupathy
Dauberman, Judy
Poulose, A. J.
Van Bielen, Jan
Power, Scott
Shew, Brian
Gray, Greg
Norton, Shirley
Van Kimmenade, Anita
Jarnagin, Alisha
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Issue Date
1991
Submitted date
2024-03-13
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Abstract
A lipase from a species of Pseudomonas has been cloned and expressed in E. coli. Variants of this lipase have been generated, using sitespecific mutagenesis, that have significantly altered k,,,, K, and substrate specificity. We have undertaken to determine the threedimensional structure of this enzyme using X-ray crystallography. Crystals have been obtained and these crystals diffract to 2.5 Angstrom resolution. We are now in the process of evaluating heavy atom derivatives to be used to improve the phase information used to calculate electron density maps.
Citation
Lipases : structure, mechanism and genetic engineering, 71 - 76
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
EISSN
ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International