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A NOVEL ENZYMATIC ASSAY METHOD FOR HYPOXANTHINE
Kim, Jong Min Suzuki, Masayasu Schmid, Rolf D.
Kim, Jong Min
Suzuki, Masayasu
Schmid, Rolf D.
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
We developed a highly sensitive and rapid enzymatic assay for hypoxanthine with a Clark oxygen electrode as sensor. In the presence of sodium sulfite, oxidation of hypoxanthine by milk xanthine oxidase caused very rapid and increased oxygen consumption in excess of the stoichiometric requirement for hypoxanthine oxidation. Hypoxanthine from 0.5 to 10 uM can be assayed within a few minutes by addition of 25 mM sodium sulfite to the reaction mixture. This assay proved to be more than 10 times more sensitive and much more rapid than control method without sulfite.
Citation
Biosensors : applications in medicine, environmental protection and process control, 425 - 428
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International