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High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.

Jäger, Volker
; ; 
Wagner, Andreas
; 
Weber, Susanne
; 
Hust, Michael
; 
Frenzel, André
; 
Schirrmann, Thomas
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2013
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Abstract
The demand of monospecific high affinity binding reagents, particularly monoclonal antibodies, has been steadily increasing over the last years. Enhanced throughput of antibody generation has been addressed by optimizing in vitro selection using phage display which moved the major bottleneck to the production and purification of recombinant antibodies in an end-user friendly format. Single chain (sc)Fv antibody fragments require additional tags for detection and are not as suitable as immunoglobulins (Ig)G in many immunoassays. In contrast, the bivalent scFv-Fc antibody format shares many properties with IgG and has a very high application compatibility.
Citation
High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. 2013, 13:52 BMC Biotechnol.
Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
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Journal
BMC biotechnology
URI
http://hdl.handle.net/10033/615999
DOI
10.1186/1472-6750-13-52
PubMed ID
23802841
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Article
Language
en
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1472-6750
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publications of the research group recombinant protein expression (RPEX)