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TERTIARY STRUCTURE OF XYLANASE AND ESTIMATION OF ACTIVE SITES BY SITE DIRECTED MUTAGENESIS
Okada, Hirosuke
Okada, Hirosuke
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Issue Date
1989
Submitted date
2023-11-03
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Abstract
The nucleotide sequence of xylanase gene(xynA) of Bacillus pumilus IPO, a hyperproducer of xylanase, was determined and the amino acid sequence was deduced from it. Xylanase is produced as a preenzyme consisting of the mature enzyme of 201 amino acid residues and a signal peptide of 27 residues. Xylanase was analyzed by X-ray crystallography at the level of 2.2 A resolution. It is consisted with two domains, smaller and larger, between two a crevasse suitable to accept xylan molecule was observed. The mutant xylanases obtained by site directed mutagenesis having amino acid alteration; Glu93-Ser93 and Glul82_Asp182 had no catalytic activity (less than 1/10,000).
Citation
Advances in protein design, 81 - 86
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 12
ISSN
0930-4320
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ISBN
3527280243
0895739534
0895739534
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Attribution-NonCommercial-ShareAlike 4.0 International