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ENZYMEKINETICS OF LIPOLYSIS: Lipase inhibition by proteins
Verger, R. Riviere, C. Moreau, H. Gargouri, Y. Rogalska, E. Nury, S. Moulin, A. Ferrato, F. Ransac, S. Carriere, F.
Verger, R.
Riviere, C.
Moreau, H.
Gargouri, Y.
Rogalska, E.
Nury, S.
Moulin, A.
Ferrato, F.
Ransac, S.
Carriere, F.
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Issue Date
1991
Submitted date
2024-03-13
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Abstract
Apart from their general biological significance, lipolytic enzymes play an increasingly important role in biotechnology: clinical medicine, pharmacology, nutrition, food andoil technology. Lipids constitute a large part of the biomassof the hearth andlipolytic enzymes play an essential role in the metabolic turnoverofthese lipids. Phospholipids and glycolipids constitute fundamental and universal structural elements of biological membranes. Onthe other hand, triglycerides constitute the main energy reservoir of higher animals. Lipases are required in the lipid transfer from one organism to another. Forthe biochemist, perhaps the most importantand fascinating aspectof lipolytic enzymesis the unique physicochemical character ofthe reactions they catalyze. These enzymesare perfectly water soluble andact very efficiently on water insoluble lipidic substrates which spontaneously self organize in water as monomolecular films, bilayers, liposomes, emulsions or micelles. This catalysis is essentially occurring at the lipid/waterinterface. Conventional enzymekineticsis oflittle relevance under these heterogeneous conditions. The development ofan "interfacial enzymology" would be of general interest forall biological sciences because in nature most enzymatic reactions take place at membranes. Thereare at least 3 major reasonsfor using lipid monolayers as substratesfor lipolytic enzymes: 1. The technique is highly sensitive and very little lipid is needed to obtain kinetic measurements. 2. A rather common observation reported by many authors working onthekinetics oflipolytic enzymesis the presence of a lag period in the hydrolysis of both emulsions, liposomes, micelles and monolayers. Such studies should preferably be done on monolayers ofshort-chain lipids where the perturbing influence of increasing amounts of reaction products can be minimized. 3. Probably the most important reason fundamentally is the possibility of varying the "quality of interface" determined by the nature ofthe lipids forming the monolayer, the orientation of the molecules, molecular and charge density, water structure, fluidity, etc...
Citation
Lipases : structure, mechanism and genetic engineering, 105 - 116
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International