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Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Zühlke, Daniela Dörries, Kirsten Bernhardt, Jörg Maaß, Sandra Muntel, Jan Liebscher, Volkmar Pané-Farré, Jan Riedel, Katharina Lalk, Michael Völker, Uwe
Zühlke, Daniela
Dörries, Kirsten
Bernhardt, Jörg
Maaß, Sandra
Muntel, Jan
Liebscher, Volkmar
Pané-Farré, Jan
Riedel, Katharina
Lalk, Michael
Völker, Uwe
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Abstract
Absolute protein quantification was applied to follow the dynamics of the cytoplasmic proteome of Staphylococcus aureus in response to long-term oxygen starvation. For 1,168 proteins, the majority of all expressed proteins, molecule numbers per cell have been determined to monitor the cellular investments in single branches of bacterial life for the first time. In the presence of glucose the anaerobic protein pattern is characterized by increased amounts of glycolytic and fermentative enzymes such as Eno, GapA1, Ldh1, and PflB. Interestingly, the ferritin-like protein FtnA belongs to the most abundant proteins during anaerobic growth. Depletion of glucose finally leads to an accumulation of different enzymes such as ArcB1, ArcB2, and ArcC2 involved in arginine deiminase pathway. Concentrations of 29 exo- and 78 endometabolites were comparatively assessed and have been integrated to the metabolic networks. Here we provide an almost complete picture on the response to oxygen starvation, from signal transduction pathways to gene expression pattern, from metabolic reorganization after oxygen depletion to beginning cell death and lysis after glucose exhaustion. This experimental approach can be considered as a proof of principle how to combine cell physiology with quantitative proteomics for a new dimension in understanding simple life processes as an entity.
Citation
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. 2016, 6:28172 Sci Rep
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PubMed ID
PubMed Central ID
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Article
Language
en
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ISSN
2045-2322