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SYNTHESIS AND CHARACTERIZATION OF A SET OF FOUR DODECADEOXYRIBONUCLEOSIDE UNDECAPHOSPHATES CONTAINING 0°-METHYLGUANINE OPPOSITE ADENINE, CYTOSINE, GUANINE, AND THYMINE
Gaffney, B. L. Marky, L. A. Jones, R. A.
Gaffney, B. L.
Marky, L. A.
Jones, R. A.
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Issue Date
1987
Submitted date
2023-04-26
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Abstract
A set of four self-complementary dodecanucleoside undecaphosphates, d[CGNGAATTC(O6Me)GCG] (1), where N = A, C, G, or T, has been synthesized by a phosphoramidite procedure. Each sequence forms a stable duplex, with a Im between 19 and 26°C lower than the Tm Of the "parent" molecule d(CGCGAATTCGCG). The lowest melting sequence is the N=T molecule; the overall order is N = C>A>G> T. Enus 0°-met hylation of guanine creates a region of localized instability in DNA regardless of the base opposite the lesion.
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Chemical synthesis in molecular biology, 75 ff
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Book chapter
conference paper
conference paper
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en
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GBF Monographs, Vol. 8
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0930-4320
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Attribution-NonCommercial-ShareAlike 4.0 International