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INVESTIGATIONS ON GLUCOSE BIOSENSORS USING LANGMUIR-BLODGETT TECHNIQUESFOR THE IMMOBILIZATION OF DERIVATIZED GLUCOSE OXIDASE
Dremel, Bernd A. A. Kalisz, Henryk M. Schaffar, B. P. H. Draxler, S. Lippitsch, M. E. Schmid, Rolf D. Wolfbeis, Otto S.
Dremel, Bernd A. A.
Kalisz, Henryk M.
Schaffar, B. P. H.
Draxler, S.
Lippitsch, M. E.
Schmid, Rolf D.
Wolfbeis, Otto S.
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Issue Date
1992
Submitted date
2024-04-17
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Abstract
Anovel method for the detection of glucose using modified glucose oxidase on Langmuir-Blodgett films is presented. A new polymer Langmuir-Blodgett film was used to immobilize modified glucose oxidase together with an oxygen sensitive fluorescent indicator. Five different glucose oxidase preparations were investigated: native, degtycosylated, N-palmitoy!-modified, periodate oxidized and y-carbodiimide treated. The oxygen consumption in the prescence of glucose was measured via dynamic quenchingof the fluorescence of the oxygen sensitive indicator.
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Biosensors : fundamentals, technologies and applications, 259 - 262
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International