Loading...
Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation.
Kirschning, Carsten J Dreher, Stefan Maass, Björn Fichte, Sylvia Schade, Jutta Köster, Mario Noack, Andreas Lindenmaier, Werner Wagner, Hermann Böldicke, Thomas
Kirschning, Carsten J
Dreher, Stefan
Maass, Björn
Fichte, Sylvia
Schade, Jutta
Köster, Mario
Noack, Andreas
Lindenmaier, Werner
Wagner, Hermann
Böldicke, Thomas
Files
Loading...
Open Access publication
Adobe PDF, 5.11 MB
Other Titles
Abstract
Toll-like receptor (TLR) 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs) of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (alphaT2ib) which was generated from an antagonistic monoclonal antibody (mAb) towards human and murine TLR2 (T2.5) to inhibit the function of TLR2. alphaT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly4Ser)3 amino acid sequence.
Citation
Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation. 2010, 10:31 BMC Biotechnol.
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1472-6750