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BIOSENSORS WITH OXIDOREDUCTASES AND INTEGRATED COENZYME OR MEDIATOR RECYCLING
Schmidt, H.-L. Schuhmann, W. Medina, R. Uhe, B. Hämmerle, M. Gutberlet, F.
Schmidt, H.-L.
Schuhmann, W.
Medina, R.
Uhe, B.
Hämmerle, M.
Gutberlet, F.
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Issue Date
1992
Submitted date
2024-04-10
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Adobe PDF, 4.44 MB
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Abstract
Most amperometric enzyme electrodes demand integrated coenzymes and/or mediators for the transfer of redox equivalents between protein and transducer. Thefree diffusibility required for this function of the mediators implies simultaneously their leaking from the electrode chamber. Approaches to overcomethis problem, aiming on the construction of compact independent oxidoreductase electrodes are reportedin this contribution. first attempt is based on the covalent binding of oxidoreductases on various conducting supports. Independent from the material and the binding method no direct electron transfer was obtained in any case. A subsequent immobilization of mediators through long spacersis therefore envisaged. In another approach, the functionalization of conducting surfaces with orientated mediators boundthrough conducting spacersis investigated, aiming on the subsequentaffinant binding of coenzymes. The most successful and promising way seemsto bind mediators or coenzymes through spacers to the enzymes themselves and to immobilize these complexes onto or within functionalized electrode surfaces.
Citation
Biosensors : fundamentals, technologies and applications, 97 - 105
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
EISSN
ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International