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STEREOSELECTIVE DETERMINATIONOF L- AND D-AMINO ACIDS IN SERUM BY THEUSE OF IMMOBILIZED ENZYME REACTORSIN FIA

Marko-Varga, György
; 
Dominguez, Elena
; 
Carlsson, Marie
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
A flow injection method containing immobilized enzyme reactors (IMERs) ; L-amino acid oxidase (L-AAO), D-aminoacid oxidase (D-AAO)and Horse radish peroxidase (HRP) for the determination of L-and D-amino acids has be en developed. Enzymatic conversion efficiencies for twenty L-and D-amino acids are given. The linear range of the detecteable amino acids was around two orders of magnitude. The sample frequency was 19 h” with a sample volume of 50 yl. The methodis used for the stereoselective determination of L-and D-aminoacids in the presence of L-aminoacids in serum.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 165 - 168
Affiliation
1) Departmentof Analytical Chemistry, 2) Departmentof Applied Microbiology, University of Lund, P.O.Box 124, S-221 00 Lund Sweden
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 1991
URI
http://hdl.handle.net/10033/623628
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Type
Book chapter
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
EISSN
ISBN
3527282491
1560811218
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Attribution-NonCommercial-ShareAlike 4.0 International
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