Loading...
In-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs.
Bielecki, Piotr Puchałka, Jacek Wos-Oxley, Melissa L Loessner, Holger Glik, Justyna Kawecki, Marek Nowak, Mariusz Tümmler, Burkhard Weiss, Siegfried dos Santos, Vítor A P Martins
Bielecki, Piotr
Puchałka, Jacek
Wos-Oxley, Melissa L
Loessner, Holger
Glik, Justyna
Kawecki, Marek
Nowak, Mariusz
Tümmler, Burkhard
Weiss, Siegfried
dos Santos, Vítor A P Martins
Files
Loading...
OPen Access publication
Adobe PDF, 1.43 MB
Other Titles
Abstract
Pseudomonas aeruginosa is a threatening, opportunistic pathogen causing disease in immunocompromised individuals. The hallmark of P. aeruginosa virulence is its multi-factorial and combinatorial nature. It renders such bacteria infectious for many organisms and it is often resistant to antibiotics. To gain insights into the physiology of P. aeruginosa during infection, we assessed the transcriptional programs of three different P. aeruginosa strains directly after isolation from burn wounds of humans. We compared the programs to those of the same strains using two infection models: a plant model, which consisted of the infection of the midrib of lettuce leaves, and a murine tumor model, which was obtained by infection of mice with an induced tumor in the abdomen. All control conditions of P. aeruginosa cells growing in suspension and as a biofilm were added to the analysis. We found that these different P. aeruginosa strains express a pool of distinct genetic traits that are activated under particular infection conditions regardless of their genetic variability. The knowledge herein generated will advance our understanding of P. aeruginosa virulence and provide valuable cues for the definition of prospective targets to develop novel intervention strategies.
Citation
In-vivo expression profiling of Pseudomonas aeruginosa infections reveals niche-specific and strain-independent transcriptional programs. 2011, 6 (9):e24235 PLoS ONE
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1932-6203