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Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen.
Carpentier, Arnaud Nimgaonkar, Ila Chu, Virginia Xia, Yuchen Hu, Zongyi Liang, T Jake
Carpentier, Arnaud
Nimgaonkar, Ila
Chu, Virginia
Xia, Yuchen
Hu, Zongyi
Liang, T Jake
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2016-03-29
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Abstract
The establishment of protocols to differentiate human pluripotent stem cells (hPSCs) including embryonic (ESC) and induced pluripotent (iPSC) stem cells into functional hepatocyte-like cells (HLCs) creates new opportunities to study liver metabolism, genetic diseases and infection of hepatotropic viruses (hepatitis B and C viruses) in the context of specific genetic background. While supporting efficient differentiation to HLCs, the published protocols are limited in terms of differentiation into fully mature hepatocytes and in a smaller-well format. This limitation handicaps the application of these cells to high-throughput assays. Here we describe a protocol allowing efficient and consistent hepatic differentiation of hPSCs in 384-well plates into functional hepatocyte-like cells, which remain differentiated for more than 3weeks. This protocol affords the unique opportunity to miniaturize the hPSC-based differentiation technology and facilitates screening for molecules in modulating liver differentiation, metabolism, genetic network, and response to infection or other external stimuli.
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Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen. 2016, 16 (3):640-650 Stem Cell Res
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1876-7753