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CLONING AND EXPRESSION OF INDUSTRIALLY IMPORTANT FUNGALLIPASES
Boel, Esper Huge-Jensen, Birgitte Wöldike, Helle F. Gormsen, Erik Christensen, Mogens Andreasen, Frank Thim, Lars
Boel, Esper
Huge-Jensen, Birgitte
Wöldike, Helle F.
Gormsen, Erik
Christensen, Mogens
Andreasen, Frank
Thim, Lars
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Issue Date
1991
Submitted date
2024-03-20
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Abstract
Triglyceride lipases from various organisms have a numberof potential industrial applications exemplified by their use in flavour enhancement, in production of esters and specialty fats, and in household detergents. We have cloned and expressed two fungallipases: the 1,3-positional specific lipase from Rhizomucor miehei (1,2), commercialized as Lipozyme™, and lipase from Humicola lanuginosa commercialized for use in household detergents (Lipolase™). Production of these lipases and other enzymesforindustrial application has necessitated the developmentof an efficient recombinant expression system. We have focusedour efforts on the development of Aspergillus as a recombinant host system for this purpose. Aspergillus has the capacity to secrete large amounts of active hydrolytic enzymes such as for example glucoamylases and amylases. Cloning of strong promoters from the corresponding genes (3,4) and development of a transformation- and selection system (4) has allowed expression from heterologous cDNA genesin Aspergillus oryzae. As first attempt to obtain heterologous expression we transferred an aspartic proteinase cDNA (5) from Rhizomucor miehei into A. oryzae and obtained high levels of secreted, active and correctly processed enzyme (4). These experiments have later been extended to fungal triglyceride lipases (6,7).
Citation
Lipases : structure, mechanism and genetic engineering, 207 - 219
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International