Loading...
Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense.
Sommer, Roman Makshakova, Olga N Wohlschlager, Therese Hutin, Stephanie Marsh, May Künzler, Markus Varrot, Annabelle
Sommer, Roman
Makshakova, Olga N
Wohlschlager, Therese
Hutin, Stephanie
Marsh, May
Künzler, Markus
Varrot, Annabelle
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2018-03-06
Submitted date
Files
Loading...
original manuscript
Adobe PDF, 4.28 MB
Loading...
supplemental material
Adobe PDF, 4.58 MB
Other Titles
Abstract
Innate immunity is the first line of defense against pathogens and predators. To initiate a response, it relies on the detection of invaders, where lectin-carbohydrate interactions play a major role. O-Methylated glycans were previously identified as non-self epitopes and conserved targets for defense effector proteins belonging to the tectonin superfamily. Here, we present two crystal structures of Tectonin 2 from the mushroom Laccaria bicolor in complex with methylated ligands, unraveling the molecular basis for this original specificity. Furthermore, they revealed the formation of a ball-shaped tetramer with 24 binding sites distributed at its surface, resembling a small virus capsid. Based on the crystal structures, a methylation recognition motif was identified and found in the sequence of many tectonins from bacteria to human. Our results support a key role of tectonins in innate defense based on a distinctive and conserved type of lectin-glycan interaction.
Citation
Crystal Structures of Fungal Tectonin in Complex with O-Methylated Glycans Suggest Key Role in Innate Immune Defense. 2018, 26 (3):391-402.e4 Structure
Publisher
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1878-4186