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CRYSTALLISATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF LIPASES
Cleasby, Anne
Cleasby, Anne
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Issue Date
1991
Submitted date
2024-03-13
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Abstract
Lipases from two Pseudomonas species have been crystallised in various forms some of which appear suitable for a detailed structural analysis by X-ray crystallography. All crystals were grown using the method of vapour diffusion by hanging drop or sitting drop, at 4C or 15C. Different crystal forms tended to appear at the two temperatures chosen for the crystallisation trials. The two lipases used came from Pseudomonas Amano, and Pseudomonas Glumae. Crystals appeared under different conditions for the two enzymes, but at least one crystal form of each diffracted to greater than 3.0A. The work on P. Amano has as yet gone no further than the ascertation of conditions for crystallisation to occur, but space groups have been determined for three crystal forms of P. Glumae. The first crystals that were tested proved to be trigonal, but were unsuitable for detailed investigation, the two others were both possible candidates for high resolution studies. One form is tetragonal, crystallising in space group P422, the other is orthorhombic, the crystals growing in space group P222, It is on this second form that subsequent structural analysis has been concentrated.
Citation
Lipases : structure, mechanism and genetic engineering, 77 - 80
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International