Thumbnail Image
Publication

Cytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein.

Wolters, Manuel
; 
Boyle, Erin C
; 
Lardong, Kerstin
; 
Trülzsch, Konrad
; 
Steffen, Anika
; 
Rottner, Klemens
; 
Ruckdeschel, Klaus
; 
Aepfelbacher, Martin
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2013-08-09
Submitted date
Show all metadata
Files
Thumbnail Image
original manuscript
Adobe PDF, 338.54 KB
Thumbnail Image
supplementary materials
Adobe PDF, 754.6 KB
Thumbnail Image
figure 1
JPEG, 6.76 MB
Thumbnail Image
figure 2
JPEG, 1.66 MB
Thumbnail Image
figure 3
JPEG, 2.18 MB
Other Titles
Abstract
Pathogenic Yersinia spp. translocate the effectors YopT, YopE, and YopO/YpkA into target cells to inactivate Rho family GTP-binding proteins and block immune responses. Some Yersinia spp. also secrete the Rho protein activator cytotoxic necrotizing factor-Y (CNF-Y), but it has been unclear how the bacteria may benefit from Rho protein activation. We show here that CNF-Y increases Yop translocation in Yersinia enterocolitica-infected cells up to 5-fold. CNF-Y strongly activated RhoA and also delayed in time Rac1 and Cdc42, but when individually expressed, constitutively active mutants of Rac1, but not of RhoA, increased Yop translocation. Consistently, knock-out or knockdown of Rac1 but not of RhoA, -B, or -C inhibited Yersinia effector translocation in CNF-Y-treated and control cells. Activation or knockdown of Cdc42 also affected Yop translocation but much less efficiently than Rac. The increase in Yop translocation induced by CNF-Y was essentially independent of the presence of YopE, YopT, or YopO in the infecting Yersinia strain, indicating that none of the Yops reported to inhibit translocation could reverse the CNF-Y effect. In summary, the CNF-Y activity of Yersinia strongly enhances Yop translocation through activation of Rac.
Citation
Cytotoxic necrotizing factor-y boosts yersinia effector translocation by activating rac protein. 2013, 288 (32):23543-53 J. Biol. Chem.
Affiliation
From the Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, 20246 Hamburg, Germany.
Publisher
Journal
The Journal of biological chemistry
URI
http://hdl.handle.net/10033/302501
DOI
10.1074/jbc.M112.448662
PubMed ID
23803609
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
1083-351X
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Archived with thanks to The Journal of biological chemistry
Collections
Publications of RG Cytoskeleton Dynamics (CYD)