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Cyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice.
Skrnjug, Ivana Guzmán, Carlos Alberto Ruecker, Christine
Skrnjug, Ivana
Guzmán, Carlos Alberto
Ruecker, Christine
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Abstract
The recently discovered mammalian enzyme cyclic GMP-AMP synthase produces cyclic GMP-AMP (cGAMP) after being activated by pathogen-derived cytosolic double stranded DNA. The product can stimulate STING-dependent interferon type I signaling. Here, we explore the efficacy of cGAMP as a mucosal adjuvant in mice. We show that cGAMP can enhance the adaptive immune response to the model antigen ovalbumin. It promotes antigen specific IgG and a balanced Th1/Th2 lymphocyte response in immunized mice. A characteristic of the cGAMP-induced immune response is the slightly reduced induction of interleukin-17 as a hallmark of Th17 activity - a distinct feature that is not observed with other cyclic di-nucleotide adjuvants. We further characterize the innate immune stimulation activity in vitro on murine bone marrow-derived dendritic cells and human dendritic cells. The observed results suggest the consideration of cGAMP as a candidate mucosal adjuvant for human vaccines.
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Cyclic GMP-AMP Displays Mucosal Adjuvant Activity in Mice. 2014, 9 (10):e110150 PLoS ONE
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1932-6203
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