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BIOSENSORS ON-LINE IN HPLC
Hippe, Helga Stadler, Herbert
Hippe, Helga
Stadler, Herbert
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
Analysis in clinical chemistry, food chemistry or biochemistry are often concerned with the difficulty to determine special substances with high sensitivity (e.g. in the pmole-range). Often the commonly known methods for detection are not sensitive enough or complicated sample preparations are necessary. To solve such problems in analysis we have developed on-line-Biosensors for HPLC. The Biosensor in this system is defined by two parts: immobilized enzymes creating H202 and a Pt-working electrode in an electrochemical detector. We present here two examples of the method. Sensitive and rapid determination of acetylcholine and choline and of hypoxanthine and xanthine. By combining the high resolving power of HPLC with the specificity of immobilized enzymes and the high sensitivity of electrochemical detection, HPLC/Biosensor-technology is a simple and economic method for many problens in analysis and will have widespread further applications.
Citation
Biosensors : applications in medicine, environmental protection and process control, 289 - 292
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Type
Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
EISSN
ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International