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Molecular profiling of tissue biopsies reveals unique signatures associated with streptococcal necrotizing soft tissue infections

Thänert, Robert
; 
Itzek, Andreas
; 
Hoßmann, Jörn
; 
Hamisch, Domenica
; 
Madsen, Martin Bruun
; 
Hyldegaard, Ole
; 
Skrede, Steinar
; 
Bruun, Trond
; 
Norrby-Teglund, Anna
; ... show 1 more
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Issue Date
2019-08-26
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Abstract
Necrotizing soft tissue infections (NSTIs) are devastating infections caused by either a single pathogen, predominantly Streptococcus pyogenes, or by multiple bacterial species. A better understanding of the pathogenic mechanisms underlying these different NSTI types could facilitate faster diagnostic and more effective therapeutic strategies. Here, we integrate microbial community profiling with host and pathogen(s) transcriptional analysis in patient biopsies to dissect the pathophysiology of streptococcal and polymicrobial NSTIs. We observe that the pathogenicity of polymicrobial communities is mediated by synergistic interactions between community members, fueling a cycle of bacterial colonization and inflammatory tissue destruction. In S. pyogenes NSTIs, expression of specialized virulence factors underlies infection pathophysiology. Furthermore, we identify a strong interferon-related response specific to S. pyogenes NSTIs that could be exploited as a potential diagnostic biomarker. Our study provides insights into the pathophysiology of mono- and polymicrobial NSTIs and highlights the potential of host-derived signatures for microbial diagnosis of NSTIs.
Citation
Nat Commun. 2019 Aug 26;10(1):3846. doi: 10.1038/s41467-019-11722-8.
Affiliation
HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.
Publisher
Nature
Journal
Nature communications
URI
http://hdl.handle.net/10033/621933
DOI
10.1038/s41467-019-11722-8
PubMed ID
31451691
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Article
Language
en
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2041-1723
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Attribution-NonCommercial-ShareAlike 4.0 International
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publications of the work group microbial interactions and processes (MINP)
publications of the research group immunology of infection ([HZI]INI)