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Multisystem combined uranium resistance mechanisms and bioremediation potential of Stenotrophomonas bentonitica BII-R7: Transcriptomics and microscopic study
Pinel-Cabello, M. Jroundi, F. López-Fernández, M. Geffers, R. Jarek, M. Jauregui, R. Link, A. Vílchez-Vargas, R. Merroun, M. L.
Pinel-Cabello, M.
Jroundi, F.
López-Fernández, M.
Geffers, R.
Jarek, M.
Jauregui, R.
Link, A.
Vílchez-Vargas, R.
Merroun, M. L.
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2021-02-05
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Abstract
The potential use of microorganisms in the bioremediation of U pollution has been extensively described. However, a lack of knowledge on molecular resistance mechanisms has become a challenge for the use of these technologies. We reported on the transcriptomic and microscopic response of Stenotrophomonas bentonitica BII-R7 exposed to 100 and 250 μM of U. Results showed that exposure to 100 μM displayed up-regulation of 185 and 148 genes during the lag and exponential phases, respectively, whereas 143 and 194 were down-regulated, out of 3786 genes (>1.5-fold change). Exposure to 250 μM of U showed up-regulation of 68 genes and down-regulation of 290 during the lag phase. Genes involved in cell wall and membrane protein synthesis, efflux systems and phosphatases were up-regulated under all conditions tested. Microscopic observations evidenced the formation of U-phosphate minerals at membrane and extracellular levels. Thus, a biphasic process is likely to occur: the increased cell wall would promote the biosorption of U to the cell surface and its precipitation as U-phosphate minerals enhanced by phosphatases. Transport systems would prevent U accumulation in the cytoplasm. These findings contribute to an understanding of how microbes cope with U toxicity, thus allowing for the development of efficient bioremediation strategies.
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Journal of hazardous materials 2021 Jan 5 DOI:10.1016/j.jhazmat.2020.123858.
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en
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03043894
EISSN
18733336
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Ministerio de Educación, Cultura y Deporte
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Attribution-NonCommercial-ShareAlike 4.0 International