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GLYCOSYLATION OF THE INFLUENZA VIRUS HEMAGGLUTININ IN INSECT CELLS
Klenk, Hans-Dieter Kuroda, Kazumichi Gröner, Albrecht Doerfler, Walter Geyer, Hildegard Geyer, Rudolf
Klenk, Hans-Dieter
Kuroda, Kazumichi
Gröner, Albrecht
Doerfler, Walter
Geyer, Hildegard
Geyer, Rudolf
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Issue Date
1991
Submitted date
2024-02-20
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Abstract
The hemagglutinin of influenza virus grown in chick embryo cells has N-glycosidic carbohydrate side chains which are of the complex type at most and of the oligomannosidic type at a few attachmentsites. When the hemagglutinin was expressed in insect cells using a baculovirus vector, it was found that these cells have the capacity to trim N-glycans to ManzGlcNAc, cores and to further process these by the addition of fucose to Manz[Fuc]GleNAc,. Thus, the complex oligosaccharides found on hemagglutinin from vertebrate hosts are replaced on hemagglutinin derived from insect cells by small truncated side chains. The effects of the truncated oligosaccharides on maturation of the hemagglutinin and onits biological functions will be discussed.
Citation
Protein glycosylation, 97 - 104
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 15
ISSN
0930-4320
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ISBN
1560811846
3527283676
3527283676
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Attribution-NonCommercial-ShareAlike 4.0 International