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CLONING, SEQUENCING AND REGULATION OF THE LIPASE GENE FROM PSEUDOMONASSP. M-12-33
Nakanishi, Yuji Watanabe, Hidemi Washizu, Kinya Narahashi, Yoshiko Kurono, Yoshiaki
Nakanishi, Yuji
Watanabe, Hidemi
Washizu, Kinya
Narahashi, Yoshiko
Kurono, Yoshiaki
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Issue Date
1991
Submitted date
2024-03-27
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Abstract
Thelipase gene from Pseudomonas sp. M-12-33 was cloned. The cloned DNA was 2.9 Kb in length, which was essential for production of the lipase and contained two open reading frames, lipA and lipX. The lipA gene was supposed to comprise 1092 nucleotides and give a preproprotein of 364 aminoacids which was then processed to a mature lipase protein of 320 amino acids. On the other hand, the lipX gene was assumedto code a protein of 344 amino acids concerned with someregulation of the enzyme production.
Citation
Lipases : structure, mechanism and genetic engineering, 263 - 266
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
3527283323
156081165X
156081165X
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Attribution-NonCommercial-ShareAlike 4.0 International