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Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory.
Dammeyer, Thorben Steinwand, Miriam Krüger, Sarah-C Dübel, Stefan Hust, Michael Timmis, Kenneth N
Dammeyer, Thorben
Steinwand, Miriam
Krüger, Sarah-C
Dübel, Stefan
Hust, Michael
Timmis, Kenneth N
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Abstract
Recombinant antibody fragments have a wide range of applications in research, diagnostics and therapy. For many of these, small fragments like single chain fragment variables (scFv) function well and can be produced inexpensively in bacterial expression systems. Although Escherichia coli K-12 production systems are convenient, yields of different fragments, even those produced from codon-optimized expression systems, vary significantly. Where yields are inadequate, alternative production systems are needed. Pseudomonas putida strain KT2440 is a versatile biosafety strain known for good expression of heterologous genes, so we have explored its utility as a cell factory for production of scFvs.
Citation
Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. 2011, 10:11 Microb. Cell Fact.
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PubMed ID
PubMed Central ID
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Article
Language
en
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ISSN
1475-2859