Loading...
New terpenoids from the fermentation broth of the edible mushroom .
Surup, Frank Hennicke, Florian Sella, Nadine Stroot, Maria Bernecker, Steffen Pfütze, Sebastian Rühl, Martin
Surup, Frank
Hennicke, Florian
Sella, Nadine
Stroot, Maria
Bernecker, Steffen
Pfütze, Sebastian
Rühl, Martin
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2019-01-01
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 421.03 KB
Other Titles
Abstract
The strophariaceous basidiomycete Cyclocybe aegerita (synonyms Agrocybe aegerita and A. cylindracea) is one of the most praised cultivated edible mushrooms and is being cultivated at large scale for food production. Furthermore, the fungus serves as a model organism to study fruiting body formation and the production of secondary metabolites during the life cycle of Basidiomycota. By studying the secondary metabolite profiles of C. aegerita, we found several terpenoids in submerged cultures. Aside from the main metabolite, bovistol (1), two new bovistol derivatives B and C (2, 3) and pasteurestin C as a new protoilludane (4) were isolated by preparative HPLC. Their structures were elucidated by mass spectrometry and NMR spectroscopy. The relative configurations of 2-4 were assigned by ROESY correlations, and 3JH,H coupling constants in the case of 4. Applying quantitative PCR for gene expression validation, we linked the production of bovistol and its derivatives to the respective biosynthesis gene clusters.
Citation
Beilstein J Org Chem. 2019 Apr 30;15:1000-1007. doi: 10.3762/bjoc.15.98. eCollection 2019.
Publisher
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
Description
Series/Report no.
ISSN
1860-5397
EISSN
ISBN
ISMN
Gov't Doc #
Sponsors
License
Attribution-NonCommercial-ShareAlike 4.0 International