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ADSORPTION OF DYES AND PROTEINS TO MONOLAYERS
Möbius, Dietmar Dhathathreyan, Aruna Kozarac, Zlatica Loschek, Rolf Müller, A.
Möbius, Dietmar
Dhathathreyan, Aruna
Kozarac, Zlatica
Loschek, Rolf
Müller, A.
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Issue Date
1989
Submitted date
2024-01-16
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Abstract
The reflection of light at the air/water interface is modified by formation of a monolayer. In the presence of dye molecules at the interface the reflection spectrum coincides with the absorption spectrum of the dye monolayer. Using the reflection technique the adsorption of ionic dyes from the aqueous subphase to mixed monolayers including head groups of opposite charge have been investigated. Electrostatic binding of the dye is enhanced at constant charge density if the charge distribution matches that of the adsorbate. Binding of cytochrome c to phospholipid monolayers is dominated by electrostatic interactions. The porphin chromophore of the adsorbed protein is oriented parallel to the monolayer plane.
Citation
Biosensors : applications in medicine, environmental protection and process control, 331 - 338
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 13
ISSN
0930-4320
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ISBN
3527280324
0895739550
0895739550
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Attribution-NonCommercial-ShareAlike 4.0 International