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SIMULTANEOUS FLOW INJECTION DETERMINATION OF MALATE AND ETHANOL BASED ON OXYGEN CONSUMPTION WHITH DEHYDROGENASE AND OXIDASE REACTORS
Ukeda, Hiroyuki Nakada, Yuji Matsumoto, Kiyoshi Osajima, Yutaka
Ukeda, Hiroyuki
Nakada, Yuji
Matsumoto, Kiyoshi
Osajima, Yutaka
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Issue Date
1991
Submitted date
2024-02-07
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Abstract
The simultaneous determination system of L-malate and ethanol was developed by using two enzyme reactors in parallel and a single oxygen electrode. NADH formed in the reaction of malate dehydrogenase was regenerated to NAD with dissolved oxygen using vitamin K; and diaphorase. Ethanol was determined by use of alcohol oxidase. When a sample solution was simultaneously injected to two reactors, a train of two peaks corresponding to ethanol and L-malate appeared in an FIA-gran. The peak current was linearly related to ethanol and L-malate concentration in the range 18-50 mM and 0.09-0.9 mM, respectively. The present system was applicable to the determination of ethanol and L-malate in wine.
Citation
Flow Injection Analysis (FIA) Based On Enzymes Or Antibodies, 203 - 208
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Book chapter
conference paper
conference paper
Language
en
Description
Series/Report no.
GBF monographs ; Volume 14
ISSN
0930-4320
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ISBN
1560811218
3527282491
3527282491
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Attribution-NonCommercial-ShareAlike 4.0 International