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Juxtanodin is an intrinsically disordered F-actin-binding protein.
Ruskamo, Salla Chukhlieb, Maryna Vahokoski, Juha Bhargav, Saligram Prabhakar Liang, Fengyi Kursula, Inari Kursula, Petri
Ruskamo, Salla
Chukhlieb, Maryna
Vahokoski, Juha
Bhargav, Saligram Prabhakar
Liang, Fengyi
Kursula, Inari
Kursula, Petri
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Abstract
Juxtanodin, also called ermin, is an F-actin-binding protein expressed by oligodendrocytes, the myelin-forming cells of the central nervous system. While juxtanodin carries a short conserved F-actin-binding segment at its C terminus, it otherwise shares no similarity with known protein sequences. We carried out a structural characterization of recombinant juxtanodin in solution. Juxtanodin turned out to be intrinsically disordered, as evidenced by conventional and synchrotron radiation CD spectroscopy. Small-angle X-ray scattering indicated that juxtanodin is a monomeric, highly elongated, unfolded molecule. Ensemble optimization analysis of the data suggested also the presence of more compact forms of juxtanodin. The C terminus was a strict requirement for co-sedimentation of juxtanodin with microfilaments, but juxtanodin had only mild effects on actin polymerization. The disordered nature of juxtanodin may predict functions as a protein interaction hub, although F-actin is its only currently known binding partner.
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Juxtanodin is an intrinsically disordered F-actin-binding protein. 2012, 2:899 Sci Rep
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en
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2045-2322
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