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BIOSENSORS BASED ON RECEPTORS : IMMUNOSENSORS ON THE BASIS OF LIPOPEPTIDES
Beck, W. Metzger, J. Wiesmüller, K.-H. Jung, G.
Beck, W.
Metzger, J.
Wiesmüller, K.-H.
Jung, G.
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Issue Date
1992
Submitted date
2024-04-17
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Abstract
Covalent attachment of the lipoamino acid N-palmitoyl-S-[2,3-bis(palmitoyloxy)-propyl]- [2RS]-cysteine (Pam3Cys-OH) (10) - a synthetic analogue to the N-terminal part of lipoprotein from the outer membrane of E. coli (11) - to peptidic antigens (epitopes) results in lipopeptideconjugates with interesting immunological and biophysical properties. Lipopeptides are able to activate B-lymphocytes (10,12), macrophages (13) and neutrophilic cells (14) and induce the production of specific antibodies against the antigenic peptide (15). The developmentof a fully synthetic vaccine against foot-and-mouth disease (FMDV) (16) as well as the in vivo-priming of cytotoxic T-lymphocytes (17) demonstrate the immunological power of lipopeptides. Biophysical investigations with labelled lipopeptides (18-20) show that lipopeptides have a high tendency to insert into membranes of living cells and into artificial phospholipid membranes (vesicles). The high affinity of lipopeptides to hydrophobic surfaces has been used for the coating of polystyrene microtiter plates in an assay (ELISA) for the distinction between HIV-1 and HIV-2 (21).
Citation
Biosensors : fundamentals, technologies and applications, 170 - 173
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
3527284370
1560812206
1560812206
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Attribution-NonCommercial-ShareAlike 4.0 International