Loading...
The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.
Kutle, Ivana Sengstake, Sarah Templin, Corinna Glaß, Mandy Kubsch, Tobias Keyser, Kirsten A Binz, Anne Bauerfeind, Rudolf Sodeik, Beate Čičin-Šain, Luka
Kutle, Ivana
Sengstake, Sarah
Templin, Corinna
Glaß, Mandy
Kubsch, Tobias
Keyser, Kirsten A
Binz, Anne
Bauerfeind, Rudolf
Sodeik, Beate
Čičin-Šain, Luka
Citations
Altmetric:
Advisors
Editors
Other Contributors
Issue Date
2017-11-14
Submitted date
Files
Loading...
Open Access publication
Adobe PDF, 5.07 MB
Other Titles
Abstract
Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.
Citation
The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. 2017, 7 (1):15588 Sci Rep
Publisher
Journal
PubMed ID
PubMed Central ID
Additional Links
Embedded video
Type
Article
Language
en
Description
Series/Report no.
ISSN
2045-2322