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Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.
Righetti, Francesco Nuss, Aaron M Twittenhoff, Christian Beele, Sascha Urban, Kristina Will, Sebastian Bernhart, Stephan H Stadler, Peter F Narberhaus, Franz
Righetti, Francesco
Nuss, Aaron M
Twittenhoff, Christian
Beele, Sascha
Urban, Kristina
Will, Sebastian
Bernhart, Stephan H
Stadler, Peter F
Narberhaus, Franz
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2016-06-28
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Abstract
RNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 °C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 °C, 37 °C, and 42 °C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.
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Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis. 2016, 113 (26):7237-42 Proc. Natl. Acad. Sci. U.S.A.
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en
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1091-6490