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Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems.

Pereira, Rui P A
; 
Peplies, Jörg
; 
Brettar, Ingrid
; 
Höfle, Manfred G
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2017-03-31
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Abstract
Next Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because for many questions in microbiology only a specific part of the whole microbiome is of interest. We have chosen the genus Legionella, comprising more than 20 pathogenic species, due to its high relevance for water-based respiratory infections.
Citation
Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems. 2017, 17 (1):79 BMC Microbiol.
Affiliation
Helmholtz Centre for infection research, Inhoffenstr. 7, 38124 Braunschweig, Germany.
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Journal
BMC microbiology
URI
http://hdl.handle.net/10033/620890
DOI
10.1186/s12866-017-0987-5
PubMed ID
28359254
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Article
Language
en
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1471-2180
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publications of the research group microbial diagnosis (MIDI)