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SIGNAL GENERATION AND EVALUATION IN FLUORESCENCE-BASED ON-LINE FIBER OPTIC BIOSENSORS
Kasche, V. Renken, E. Schietke, G. Ulrich, R. Bücke, R. Gnewuch, H. Jansen, K.
Kasche, V.
Renken, E.
Schietke, G.
Ulrich, R.
Bücke, R.
Gnewuch, H.
Jansen, K.
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Issue Date
1992
Submitted date
2024-04-30
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Abstract
The signal generation, size and transmission of pre-steady-state and steady-state signals in fluorescence based fiber optic biosensors for on-line use and their applicability in several flow sensor configurations are investigated. In contrast to the theory, the signal for dynamic column biosensorswith larger particles was foundto be onlyslightly higher than for a column sensorwith smaller particles. This is due to interparticle interactions, which reduce the areathat is accessible for mass transfer, on particles with a diameter below 100 pm. Additionally this system has shown that dynamic biosensors with hydrolases can not be used for the determination of the end-pointof hydrolytic processes, when the equilibrium of the substrate conversion remains below 100%. The flow of optical power in fluorescent fiber optic sensors is analyzed, multiplicative noise is identified as the dominantlimitation of sensor performance. Evanescent coupling is a promising concept for fast biosensors. The observed nonlinear relation between absorbance and concentration is explained as an optical effect, caused by multimode operation. The use of special fibers with increased surface area gives higher sensitivity, reduced nonlinearity.
Citation
Biosensors : fundamentals, technologies and applications, 265 - 274
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
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ISBN
1560812206
3527284370
3527284370
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Attribution-NonCommercial-ShareAlike 4.0 International