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A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains.
Lee, Changhan Wigren, Edvard Trček, Janja Peters, Verena Kim, Jihong Hasni, Muhammad Sharif Nimtz, Manfred Lindqvist, Ylva Park, Chankyu Curth, Ute
Lee, Changhan
Wigren, Edvard
Trček, Janja
Peters, Verena
Kim, Jihong
Hasni, Muhammad Sharif
Nimtz, Manfred
Lindqvist, Ylva
Park, Chankyu
Curth, Ute
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2015-11
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Abstract
Pseudomonas aeruginosa is a highly successful nosocomial pathogen capable of causing a wide variety of infections with clone C strains most prevalent worldwide. In this study, we initially characterize a molecular mechanism of survival unique to clone C strains. We identified a P. aeruginosa clone C-specific genomic island (PACGI-1) that contains the highly expressed small heat shock protein sHsp20c, the founding member of a novel subclass of class B bacterial small heat shock proteins. sHsp20c and adjacent gene products are involved in resistance against heat shock. Heat stable sHsp20c is unconventionally expressed in stationary phase in a wide temperature range from 20 to 42°C. Purified sHsp20c has characteristic features of small heat shock protein class B as it is monodisperse, forms sphere-like 24-meric oligomers and exhibits significant chaperone activity. As the P. aeruginosa clone C population is significantly more heat shock resistant than genetically unrelated P. aeruginosa strains without sHsp20c, the horizontally acquired shsp20c operon might contribute to the survival of worldwide-distributed clone C strains.
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A novel protein quality control mechanism contributes to heat shock resistance of worldwide-distributed Pseudomonas aeruginosa clone C strains. 2015, 17 (11):4511-26 Environ. Microbiol.
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en
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1462-2920