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CRYSTALLOGRAPHIC STUDIES OF THE PANCREATIC LIPASE/COLIPASE SYSTEM
Cambillau, Christian Bourne, Yves Chapus, Catherine Lombardo, Dominique Rovery, Mireille
Cambillau, Christian
Bourne, Yves
Chapus, Catherine
Lombardo, Dominique
Rovery, Mireille
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Issue Date
1991
Submitted date
2024-03-06
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Abstract
Crystals of a complex between porcine pancreatic lipase (50000 Da) andits cofactor, colipase (11000 Da), have been obtained. These 1/1 complex crystals are disordered, and diffract weakly. Horse pancreatic lipase has been crystallized in space group P21242), (89A,97A,145A ; pH 6.0, PEG 8000 10%, 20°C) and a 2.3 A native data set has been collected. One PCMBS derivative was not sufficient to produce a good map,andwearestill looking for other derivatives. Two crystal forms of human pancreatic lipase (purified from pancreas juice) have been obtained, different from the one reportedin thelitterature. They crystallize in the same conditions and space groupsare P2, and P1. Native data sets have beencollected.
Citation
Lipases : structure, mechanism and genetic engineering, 31 - 34
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Book chapter
conference paper
conference paper
Language
en
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Series/Report no.
GBF monographs ; Volume 16
ISSN
0930-4320
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ISBN
156081165X
3527283323
3527283323
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Attribution-NonCommercial-ShareAlike 4.0 International