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Functional analysis of colonization factor antigen I positive enterotoxigenic identifies genes implicated in survival in water and host colonization.
Abd El Ghany, Moataz Barquist, Lars Clare, Simon Brandt, Cordelia Mayho, Matthew Joffre, Enrique Sjöling, Åsa Turner, A Keith Klena, John D Kingsley, Robert A
Abd El Ghany, Moataz
Barquist, Lars
Clare, Simon
Brandt, Cordelia
Mayho, Matthew
Joffre, Enrique
Sjöling, Åsa
Turner, A Keith
Klena, John D
Kingsley, Robert A
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Abstract
Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
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Microb Genom. 2021 Jun;7(6). doi: 10.1099/mgen.0.000554.
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en
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2057-5858
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Attribution 4.0 International