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Expansion of functional personalized cells with specific transgene combinations.
Lipps, Christoph Klein, Franziska Wahlicht, Tom Seiffert, Virginia Butueva, Milada Zauers, Jeannette Truschel, Theresa Luckner, Martin Köster, Mario MacLeod, Roderick
Lipps, Christoph
Klein, Franziska
Wahlicht, Tom
Seiffert, Virginia
Butueva, Milada
Zauers, Jeannette
Truschel, Theresa
Luckner, Martin
Köster, Mario
MacLeod, Roderick
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2018-03-08
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Abstract
Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.
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Nat Commun. 2018;9(1):994. Published 2018 Mar 8. doi:10.1038/s41467-018-03408-4.
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en
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2041-1723
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Attribution-NonCommercial-ShareAlike 4.0 International