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Quantitative image analysis of microbial communities with BiofilmQ.

Hartmann, Raimo
Jeckel, Hannah
Jelli, Eric
Singh, Praveen K
Vaidya, Sanika
Bayer, Miriam
Rode, Daniel K H
Vidakovic, Lucia
Díaz-Pascual, Francisco
Fong, Jiunn C N
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2021-01-04
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Abstract
Biofilms are microbial communities that represent a highly abundant form of microbial life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space and time; microscopy and quantitative image analysis are therefore crucial for elucidating their functions. Here, we present BiofilmQ-a comprehensive image cytometry software tool for the automated and high-throughput quantification, analysis and visualization of numerous biofilm-internal and whole-biofilm properties in three-dimensional space and time.
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Nat Microbiol. 2021 Jan 4. doi: 10.1038/s41564-020-00817-4. Epub ahead of print.
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en
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2058-5276
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Attribution 4.0 International