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Preparation, characterisation and in vitro antibacterial property of ciprofloxacin-loaded nanostructured lipid carrier for treatment of Bacillus subtilis infection.
Nnamani, Petra Ugwu, Agatha Ibezim, Emmanuel Onoja, Simon Odo, Amelia Windbergs, Maike Rossi, Chiara Lehr, Claus-Michael Attama, Anthony
Nnamani, Petra
Ugwu, Agatha
Ibezim, Emmanuel
Onoja, Simon
Odo, Amelia
Windbergs, Maike
Rossi, Chiara
Lehr, Claus-Michael
Attama, Anthony
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2019-02-13
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CONTEXT: In this study, controlled ciprofloxacin (CIPRO) nanostrustructured lipid carriers of Precirol® ATO 5/Transcutol® HP (batch A) and tallow fat/Transcutol® HP (batch B) was carreid out. OBJECTIVE: The aim was to improve solubility and bioavailability of CIPRO. OBJECTIVE: Study of controlled ciprofloxacin (CIPRO) nanostructured lipid carriers of Precirol® ATO 5/Transcutol® HP (batch A) and tallow fat/Transcutol® HP (batch B). METHODS: CIPRO concentrations C1-5 (0.0, 0.2, 0.5, 0.8, and 1.0% w/w) as AC1-5 and BC1-5 were prepared by hot homogenisation and characterised by zetasizer, differential scanning calorimetry, Fourier transform infra-red spectroscopy, in vitro drug release and growth inhibitory zone diameter (IZD) on agar-seeded Bacillus subtilis. RESULTS: AC5 achieved polydispersed particles of ∼605 nm, 92% encapsulation efficiency (EE) and -28 mV similar to BC5 (∼789 nm, 91% EE, and -31 mV). Crystallinity indices (AC5 and BC5) were low at 3 and 5%, respectively. CIPRO release in AC5 was ∼98% in SGF (pH 1.2) and BC5 similarly ∼98% in SIF (pH 6.8). CONCLUSIONS: AC5 had superior growth inhibition of B. subtilis at lower concentration (1.2 µg/mL) than BC5 and CIPRO controls; hence could serve as possible sustained delivery system of CIPRO.
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en
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1464-5246
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Attribution-NonCommercial-ShareAlike 4.0 International